By Richard A. Hopkins M.D. (auth.)
A significant desire of all cardiologists and surgeons concerned with the appliance of allograft tissue to cardiac surgical reconstructions is met via this designated atlas. step-by-step, it conscientiously describes the techniques all for buying, sterilizing, and cryo-preserving allograft valves in addition to the surgical strategy utilized in left and correct ventricular outflow tract reconstructions. the entire info had to practice the reconstructions is given, together with suitable references and acceptable adaptations in procedure. first-class illustrations accompany the outline of surgical procedure. all the innovations are according to vintage techniques, yet assimilate the authors' alterations arrived at via gigantic adventure. The wealth of references, illustrations and services during this quantity make it a helpful asset for all cardiac surgeons.
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Extra info for Cardiac Reconstructions with Allograft Valves
Heslop BF, Wilson SE, Hardy BE: Antigenicity of aortic valve allografts. Ann Surg 177:301-306, 1973. 48. Buch WS, Kosek JC, Angell WW: The role of rejection and mechanical trauma on valve graft viability. ] Thorac Cardiovasc Surg 62:69&-706, 1971. 49. Rossi MA, Braile DM, Teixeira DR, Carillo SV: Calcific degeneration of pericardial valvular xenografts implanted subcutaneously in rats. I nt] C ardiolI2:331-339, 1986. SO. Levy RJ, Schoen FJ, Levy JT, et al: Biologic determinants of dystrophic calcification and osteocalcin deposition in glutaraldehyde-preserved porcine aortic valve leaflets implanted subcutaneously in rats.
HOPKINS proline, but radio labeled methionine was quickly lost from the tissue (within 1 week) and was not incorporated into extracellular products. Protein synthesis by mammalian cells includes proteins normally utilized for "housekeeping" purposes or for secretion to the extracellular environment (export). As a general statement, it may be said that two such populations of cells exist in the valve leaflet tissue of the heart. The fibroblast-fibrocyte population is involved in normal maintenance protein synthesis as well as export protein synthesis (collagen and proteoglycans).
WOLFINBARGER JR. A. HOPKINS cooling, and injury may be more closely associated with ionic imbalance and osmotic changes. Of importance to cryopreservation of heart valves is the suggestion that dimethylsulfoxide (DMSO) may actually increase sensitivity to cold shock. 6 Hence DMSO should be added to cells after the initial cooling and prior to freezing. (See ref. , glycerol and DMSO). The actual freezing of dilute aqueous solutions is governed by chemical and physical parameters rather than any biologic consideration.