By Joseph A. Vinson
content material: A survey of metabolic modifications of [delta]¹-tetrahydrocannabinol / Sumner Burstein --
GLC and HPLC analyses of cannabinoids in organic fluids and purposes / E.R. Garrett, A.J. Gouyette, and C.A. Hunt --
Detection and quantitation of tetrahydrocannabinol in physiological fluids / Monroe E. Wall, Dolores R. Brine, Joan T. Bursey, and David Rosenthal --
Quantitation of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in physique fluids through GC/CI-MS / R.L. Foltz, P.A. Clarke, B.J. Hidy, D.C.K. Lin, A.P. Graffeo, and B.A. Petersen --
development of the mass fragmentographic method for quantification of tetrahydrocannabinol in human blood plasma / Agneta Ohlsson, Stig Agurell, Jan-Erik Lindgren, and Kurt Leander --
A basic method of the research of cannabinoids from physiological resources / J. Rosenfeld --
Quantitation of [delta]⁹-tetrahydrocannabinol and its metabolites in human urine through likelihood dependent matching GC/MS / Donald E. eco-friendly, Fu-Chuan Chao, Kay O. Loeffler, and Saul L. Kanter --
HPLC research of [delta]⁹-tetrahydrocannabinol and metabolites in organic fluids / Seth R. Abbott, John R. Berg, Kay O. Loeffler, Saul Kanter, Leo E. Hollister, Joan Hawkins Abrams, Hugh L. Baras, and Reese T. Jones --
Radioimmunoassay of cannabinoid compounds / C.E. prepare dinner --
Antisera raised opposed to tetrahydrocannabinol within the radioimmunoassay of cannabinoids / J.D. Teale, Jacqueline M. Clough, L.J. King, V. Marks, P.L. Williams, and A.C. Moffat --
HPLC analyses of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in human plasma / Jimmie L. Valentine, Owen H.M. Gan, H.C. Nio, and Everett D. Thompson --
Detection and quantitation of [delta]⁹-tetrahydrocannabinol in plasma by means of dansylation and double labeling / J.M. Scherrmann, R. Bourdon, H. Hoellinger, Nguyen-Hoang Nam, and E. Fournier.
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11, 509 (1973). McCallum, Ν. Κ . , Yagen, Β . , Levy, S . , and Mechoulam, R . , Experientia 31, 520 (1975). , Agurell, S . , L i n d g r e n , J. E . , Biochem. Pharmacol. 23, 163 (1974). Fetterman, R. S . , and Turner, C . , J. Pharm. Sci. 61, 147 Turner, C . , 62, 251 and 1083 (1973). Turner, C . , Hadley, K . , and Mole, M. , J. Pharm. Sci. 63, 1872 (1974). RECEIVED December 12, 1978. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1979. 3 Detection and Quantitation of Tetrahydrocannabinol in Physiological Fluids M O N R O E E.
A -THC v a l u e s o b t a i n e d w i t h t h e LKB-9000 E I s o u r c e were i n c l o s e agreement w i t h t h e d a t a o b t a i n e d on the F i n n i g a n 3300 c h e m i c a l i o n i z a t i o n (CI) s o u r c e . ; ACS Symposium Series; American Chemical Society: Washington, DC, 1979. Figure 5. LKB 9000-EI plasma calibration curve for 11-hydroxy-A -THC 9 Comparison o f v a l u e s f o r LKB v s . F i n n i g a n EI s o u r c e a l s o showed e x c e l l e n t agreement. A -THC values i n c r e a s e d r a p i d l y d u r i n g the f i r s t 10-20 minutes, the peak v a l u e s i n t h e range o f 50-60 ng/ml c o i n c i d i n g w i t h the maximal psychomimetic a c t i v i t y .
0 mg o f A -THC by the i n t r a v e n o u s method o f Perez-Reyes e t a_l. (10) . The v o l u n t e e r s were k e p t under m e d i c a l s u p e r v i s i o n f o r 24 hours i n t h e C l i n i c a l Research U n i t o f t h e U n i v e r s i t y o f N o r t h C a r o l i n a , S c h o o l o f M e d i c i n e . B l o o d samples ( a p p r o x i m a t e l y 10 ml) were c o l l e c t e d a t p e r i o d i c i n t e r v a l s o v e r 24 h o u r s . Plasm f r o z e n i m m e d i a t e l y an analyzed.