Biophotonics: Optical Science and Engineering for the 21st by E. Gratton, S. Breusegem, N. Barry, Q. Ruan, J. Eid (auth.),

By E. Gratton, S. Breusegem, N. Barry, Q. Ruan, J. Eid (auth.), Xun Shen, Roeland Van Wijk (eds.)

Biophotonics - Optical technological know-how and Engineering for the 21st Century

- Roeland Van Wijk, PhD. and Prof. Dr. Xun Shen

Biophotonics, the technology of producing and harnessing mild photons to picture, discover and control organic fabrics, deals nice desire for the early detection of illnesses and for brand spanking new modalities of light-guided and light-activated remedies. It bargains a robust software for learning molecular occasions, reminiscent of gene expression, protein-protein interplay, spatial and temporal distribution of the molecules of organic curiosity, and plenty of chemico-physical tactics in residing cells and residing organisms.

Biophotonics - Optical technology and Engineering for the 21st Century introduces the elemental experimental and theoretical framework, the technical difficulties, and the broad box of purposes in biotechnology, biomedical engineering, engineering, medication, pharmacology, environmental technological know-how, existence sciences, and scientific sciences. Biophotonics: Optical technology and Engineering within the twenty first Century serves as an excellent relief to the study and improvement of those components integrating gentle, photonics, and organic systems.

Key subject matters include:

Fluctuation Correlation Spectroscopy in Cells: selection of Molecular Aggregation

Using GFP and agonize applied sciences for learning Signaling Mechanisms of Apoptosis in one dwelling Cell

Study on Protein-Protein interplay in unmarried residing Cells

Functional Optical Coherence Tomography: Simultaneous In Vivo Imaging of Tissue constitution and body structure

Imaging –Photo- and Sonodynamic prognosis of melanoma Mediated by means of Chemiluminescence Probes

Biophotonic research of Spontaneous Self-Organizing Oxidative approaches in Aqueous platforms

Biophoton Emission and protection structures in Plants

Biophotonics - Optical technology and Engineering for the 21st Century stands as a worthy source for complex undergraduate, graduate scholars and researchers in biomedical engineering, biotechnology, engineering, chemistry, physics, existence sciences, and scientific sciences.


Xun Shen

Professor, Institute of Biophysics, chinese language Academy of Sciences

Beijing, China

Roeland Van Wijk

Professor, division of Molecular mobile Biology, Utrecht collage,

Utrecht, The Netherlands

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Glaser, M. W. 2002. Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy. Biophys J 83(6):3177-3187. 14. , Gratton, E. 2002. Molecular brightness characterization of EGFP in vivo by fluorescencefluctuationspectroscopy. l33-44. 15. Schwille P, Meyer-Almes, F. J. and Rigler, R. 1997. Dual-colorfluorescencecross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J12: 1878-1886 16.

L. 1987. Molecular aggregation characterized by high order autocorrelation in fluorescence correlation spectroscopy. Biophys J 51:251-210. 11. T. and Gratton, E. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys J. 77(l):553-567. 12. Qian, H. L. 1990. Distribution of molecular aggregation by analysis of fluctuation moments. Proc Natl. Acad. Sci. USA. 87:5479-5483. 13. , Glaser, M. W. 2002. Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy.

Such measurements are not possible using conventional biochemical methods. In this chapter, we will give several examples to demonstrate how we use these innovative optical technologies for studying signaling mechanisms in programmed cell death. The first example is to investigate the temporal relationship between Bax translocation and the release of cytochrome c during UV-induced apoptosis. Mitochondria were known to play a central role in the apoptotic process. Specifically, releases of cytochrome c and Smac from mitochondria are the key steps that trigger the activation of caspase-9 and caspase-3 during cell death.

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