By George Klein (ed.), Sidney Weinhouse (ed.)
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It is worth noting that the only difference between transforming W M and nontransforming VMM is the altered carboxyl terminus. 4. Nuclear localization offos proteins. (A) CHOdhfr- cells transfected with pSVdhfr-fos and selected for resistance to 4 x M methotrexate, stained with TBRS. (B) The same cells stained with normal rat serum. (C) 208F rat cells transformed by recombinant clone MMV expressing clfos protein, stained with TBRS. (D) The same field as in C viewed under Nomarski optics. (E) Normal mouse amnion cells stained with TBRS.
1984), yet do not become transformed and faithfully express their phenotype (Durban and Boettiger, 1981). src-mediated block of differentiation exhibits a variable degree of stability in different cell types and experimental conditions, and thus is compatible with the retention of a residual competence to differentiate. A nuclear oncogene such as myc, on the contrary, reproducibly al- 24 STEFAN0 A L E a AND FRANC0 TAT6 ters the proliferation of many avian cell types without ostensibly affecting at least those tissue-specific functions that are compatible with an active proliferation.
Depending on the nature of the inducer, in some cases c-fos expression appears to be lineage specific (Table I). Below we describe some systems. 1 . They observed c-fos transcripts when WEH13B murine myeloid leukemia cell line was induced to macrophage-like differentiation with granulocytic colony-stimulating factors. , 1985). When human monomyelocytic cell line, HL-60, or promonocytic cell line U-937 are treated with phorbol esters (TPA), the c-fos gene is rapidly induced. Within minutes, the c-fos transcripts can be observed.