Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit

By Wayne W Umbreit

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such subject matters as environmental evaluate of biotechnological procedures and microbial differences of haloaromatic and haloaliphatic compounds.

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2 offers two significant advantages over an ordinary U-tube manometer. The most important one is that the manometer fluid can be dried and degassed in situ by heating and circulating the manometer fluid. Once degassed and evacuated the manometer can serve to check the operation of the instrument and pump, obviating the necessity of a separate vacuum gage. E. LABORATORY FABRICATED LYOPHILIZERS 1 . Glass Equipment It is hoped that a review of equipment described by various investigators will provide a basis for selecting the apparatus best suited for a specific operation.

3°C. 3°C. 00015 100 Heller (1941) 50 33 PRESERVATION OF BACTERIA BY LYOPHILIZATION TABLE I (Continued) Storage Organism and suspending fluid Temperature Time 5 % Lactose plus salts Mist,. desiccansc - - RT RT Serum Peptone Serum Skim milk Neisseria 10% Glucose and 10% peptone on starch 0 0 8-14 Months 24-30 Months RT 10 Years - 0 0 0 40 Days 10 Days - 10°C. RT RT 0 8 Months Culture added to dried plugs of peptone, dextran, and glucose RT 18 Months Mist. 5% glucose Serum Mist. desiccansc Pasteurella Equal volumes of culture and 6 % lact>ow - 0 RT RT RT RT RT 1 8-12 22-31 20-22 3 4°C.

Developed slowly. By comparison, between 1 and 15%of the Vibrio he processed in the same manner remained viable after 9 to 12 months storage at room temperature. From the above discussion it seems that the differences among bacteria are as great or greater than the differences between bacteria and yeasts. Viruses are, as a whole, more sensitive to certain drying procedures, but they are more resistant to cold shock than are bacteria. Most rickettsiae are relatively difficult to maintain as dry stock cultures although Topping (1940) and Jackson and Smadel (1951) were successful in maintaining infectivity by lyophilization.

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