A text book of practical botany 1 by Dr. Ashok Kumar Dr. Ashok Bendre

By Dr. Ashok Kumar Dr. Ashok Bendre

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Work procedure Take a small part of net-like thallus, stain in safranin, wash with water and mount in glycerine. Take care to spread the net-work in a way that cells do not get overlapped. Comments 1. The thallus is a multicellular colony forming a net-like structure. 2. Colony is a hollow and sac-like or saucer-like (saccate), cylindrical network, closed at both the ends. 3. The spaces of the reticulum are bound by five or six cells (this number varies between 3-10 cell wall nucleus pyrenoid chloroplast cytoplasm chloroplast Fig.

3. Internodal cell shows a typical cell structure. 4. Centre of the cell has a big vacuole surrounded by cytoplasm. 5. In the cytoplasm lies a single nucleus held by thin and delicate cytoplasmic strands. 6. Many discoid chloroplasts without pyrenoids are scattered in the peripheral cytoplasm. 7. The cell has an outermost, thick and ftrm cell wall. internodal cell of Fig. 2. Chara. s. s. of apex. s. of apex. Comments 1. The cell situated at the top is an apical cell. It contributes to the development of the main axis and lateral axes.

The wall of the oogonium has a small pore on one side, Known as receptive pore. Just opposite the receptive pore, protoplast of Il~c oogonium has a hyaline area-receptive spot. Uninucleate protoplast is rich in reserve food. Exercise 2 e . :;. ' Object : Study of oogonium. Work procedure Place a few filaments and observe a filament with oogonia. Stain such filaments with safranin, wash in water and mount in glycerine. Study the oogonia. Comments 1. 2. Oogonia are intercalary or terminal in position.

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